human serum (hs) with intact complement activity  (Quidel)

Journal: Infection and Immunity

Article Title: Complement C3 as a Prompt for Human Macrophage Death during Infection with Francisella tularensis Strain SCHU S4

doi: 10.1128/IAI.00424-17

Figure Lengend Snippet: Complement component C3 mediates enhanced bacterial uptake and macrophage death induction. MDM were infected with SCHU S4 (MOI = 80) in the presence of the indicated sera. *, significant differences in bacterial burden or LDH release when comparing test sera to HS at the indicated times p.i. The results shown are representative of three independent experiments. (B) Deposition of C3 peptides on SCHU S4 (8 × 107 bacteria per lane) was detected via Western blotting. Purified C3 and iC3b proteins served as markers. These results are representative of three independent experiments.

Article Snippet: Commercial human serum (HS) with intact complement activity and C3-depleted HS were purchased from either Quidel (San Diego, CA) or Complement Technology, Inc. (Tyler, TX).

Techniques: Infection, Bacteria, Western Blot, Purification

Journal: Infection and Immunity

Article Title: Complement C3 as a Prompt for Human Macrophage Death during Infection with Francisella tularensis Strain SCHU S4

doi: 10.1128/IAI.00424-17

Figure Lengend Snippet: Engagement of cell surface complement receptors is not sufficient for the induction of macrophage death, despite high cytosolic bacterial load. MDM were first infected at a high MOI with WT SCHU S4 in the presence of C3-depleted HS. This was followed by a second infection with either WT or ΔfevR SCHU S4 in the presence of HS or C3-depleted HS. Significant differences (*) in bacterial burden or LDH release are shown compared to the primary infection of WT in C3-depelted serum alone (no secondary infection). ns, not significant. The results shown are averages from 3 independent experiments in which the average MOI for the primary infection with WT in C3-depleted HS was 1,889. The average MOI in the secondary infection was 189 for WT and 97 for ΔfevR SCHU S4.

Article Snippet: Commercial human serum (HS) with intact complement activity and C3-depleted HS were purchased from either Quidel (San Diego, CA) or Complement Technology, Inc. (Tyler, TX).

Techniques: Infection